Blar i forfatter "Kuta, Piotr"

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    • Identification of the factor XII contact activation site enables sensitive coagulation diagnostics 

      Heestermans, Marco; Naudin, Clement; Mailer, Reiner K.; Konrath, Sandra; Klaetschke, Kristin; Jämsä, Anne; Frye, Maike; Deppermann, Carsten; Pula, Giordano; Kuta, Piotr; Friese, Manuel A.; Gelderblom, Mathias; Sickmann, Albert; Preston, Roger J. S.; Nofer, Jerzy-Roch; Rose-John, Stefan; Butler, Lynn; Salomon, Ophira; Stavrou, Evi X.; Renné, Thomas (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-09-22)
      Contact activation refers to the process of surface-induced activation of factor XII (FXII), which initiates blood coagulation and is captured by the activated partial thromboplastin time (aPTT) assay. Here, we show the mechanism and diagnostic implications of FXII contact activation. Screening of recombinant FXII mutants identified a continuous stretch of residues Gln317–Ser339 that was essential ...

    • Intrinsic coagulation pathway-mediated thrombin generation in mouse whole blood 

      Konrath, Sandra; Mailer, Reiner K.; Beerens, Manu; Englert, Hanna; Frye, Maike; Kuta, Piotr; Preston, Roger J. S.; Maas, Coen; Butler, Lynn; Roest, Mark; de Laat, Bas; Renné, Thomas (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-11-28)
      Calibrated Automated Thrombography (CAT) is a versatile and sensitive method for analyzing coagulation reactions culminating in thrombin generation (TG). Here, we present a CAT method for analyzing TG in murine whole blood by adapting the CAT assay used for measuring TG in human plasma. The diagnostically used artificial and physiologic factor XII (FXII) contact activators kaolin, ellagic acid ...